7/6/2023 0 Comments Cucumber blight![]() Interestingly, the DUF26 has not been attributed with any confirmed functions, however, predicted to be playing a role in regulating the three-dimensional structure of the CRKs and in the protein-protein/DNA interactions. In addition, the CRKs contain the Domain of Unknown Function 26 (DUF26) domains and thus, also known as DUF26-RLKs with a signature C-X 8-C-X 2-C motif. In plants, the signals are perceived by the extracellular domain, which subsequently passes through the transmembrane domain, and lastly transduced by the intracellular kinase domain. The CRKs possess three distinct domains, including the extracellular domain, transmembrane domain, and intracellular kinase domain. The cysteine-rich receptor-like kinases (CRKs) family belongs to the RLK superfamily and has been well-studied in plants. The receptor-like kinases (RLKs) are one such superfamily of plant proteins that functions in the perception of different stimuli and signals. These defense responses are conferred by several classes of plant proteins either by recognizing the pathogens or pathogen-associated signatures or by orchestrating the downstream signaling cascades. Plants have developed specialized strategies, such as innate immunity and systemic acquired resistance to tackle these adversities. In these stresses, pathogen infection is a major threat to plants. Plant resilience and productivity are challenged by several biotic stresses in its natural environment. Moreover, current findings provide better insights into the cucumber CRKs and their involvement in defense responses. Functional predictions and validation via expression analysis confirmed the involvement of the CsCRKs in cucumber defense response, especially against S. The results of this study identified and characterized the CRK gene family in cucumbers. Finally, the protein interaction network prediction results identified some key possible interacting partners of the CsCRKs in regulating cucumber physiological processes. Under the cucumber neck rot pathogen, Sclerotium rolfsii infection, multiple CsCRKs exhibited induced expressions at early, late, and both stages. The expression analysis of the CsCRKs by using transcriptome data and via qRT-PCR indicated their involvement in both biotic and abiotic stress responses. Functional predictions of the CsCRKs suggested their role in signaling and defense response in cucumbers. Phylogenetic analysis divided the CsCRKs into two clades along with other plant CRKs. Additionally, the gene duplication analysis of the CsCRKs yielded information on their divergence and expansion in cucumbers. Chromosome mapping of the CsCRKs revealed that 15 genes are distributed in cucumber chromosomes. ![]() ![]() sativus CRKs ( CsCRKs) have been characterized in the cucumber genome. In this study, a genome-wide characterization of the CRK family has been performed to investigate the structural and functional attributes of the cucumber CRKs under cold and fungal pathogen stress. However, the CRK family in cucumbers ( Cucumis sativus L.) has been explored to a limited extent. The plant cysteine-rich receptor-like kinases ( CRKs) are a large family having multiple roles, including defense responses under both biotic and abiotic stress.
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